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Effects of DBT on the expression of α-SMA, <t>FN1,</t> p-SMAD3 and TGF-β1 in rat lung tissue. α-SMA protein expression after (A) 28 and (B) 42 days of DBT administration. FN1 protein expression after (C) 28 and (D) 42 days of DBT administration; n = 4. (E) TGF-β1 and (F) p-SMAD3, n = 5. TGF-β1 protein expression after (G) 28 and (H) 42 days of DBT administration; n = 4 (G, H) , * P < 0.05 or ** P < 0.01; N.S. , not statistically significant, compared with the silica group.
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Effects of DBT on the expression of α-SMA, FN1, p-SMAD3 and TGF-β1 in rat lung tissue. α-SMA protein expression after (A) 28 and (B) 42 days of DBT administration. FN1 protein expression after (C) 28 and (D) 42 days of DBT administration; n = 4. (E) TGF-β1 and (F) p-SMAD3, n = 5. TGF-β1 protein expression after (G) 28 and (H) 42 days of DBT administration; n = 4 (G, H) , * P < 0.05 or ** P < 0.01; N.S. , not statistically significant, compared with the silica group.

Journal: Frontiers in Pharmacology

Article Title: Astragalus mongholicus bunge and Angelica sinensis botanical drug decoction mitigates lung inflammation through NOX4/TGF-β1/SMAD3 signaling

doi: 10.3389/fphar.2025.1565569

Figure Lengend Snippet: Effects of DBT on the expression of α-SMA, FN1, p-SMAD3 and TGF-β1 in rat lung tissue. α-SMA protein expression after (A) 28 and (B) 42 days of DBT administration. FN1 protein expression after (C) 28 and (D) 42 days of DBT administration; n = 4. (E) TGF-β1 and (F) p-SMAD3, n = 5. TGF-β1 protein expression after (G) 28 and (H) 42 days of DBT administration; n = 4 (G, H) , * P < 0.05 or ** P < 0.01; N.S. , not statistically significant, compared with the silica group.

Article Snippet: Endogenous peroxidase was incubated for 10 min, washed with phosphate-buffered saline, blocked with normal goat serum, and incubated with the following antibodies overnight at 4°C: anti-nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4) (ab154244; Abcam, Cambridge, United Kingdom), anti-TGF-β1 (346599; ZEN-bioscience), anti-α-smooth muscle actin (α-SMA) (BM3902), and anti-Fibronectin 1 (FN1) (BM4460, Boster Bio, Wuhan, China).

Techniques: Expressing